＃CentriVap Pro 位於研究流程中的關鍵角色
外泌體（Exosomes）與細胞外囊泡（Extracellular Vesicles, EVs）在免疫調節、細胞通訊、再生醫學與藥物傳遞中扮演越來越重要的角色。近年多篇研究指出，不同來源的囊泡在組成、功能與細胞攝取方式上具有關鍵差異，因此樣品處理與濃縮步驟的品質，直接影響最終研究結果的可信度與重現性。
在這些研究流程裡，「樣品濃縮」始終是關鍵且不可被忽略的前端步驟。CentriVap Pro 憑藉其穩定的真空乾燥技術、精準控溫能力與高重現性，已成為 EV/Exosome 研究團隊在前處理階段的重要工具。
＃CentriVap Pro 在 EV / Exosome 研究中的核心價值
在 EV 研究流程中，常見的關鍵步驟包含：
- 超速離心或 SEC 純化後的樣品濃縮
- 去除緩衝液中大量溶液
- 將樣品濃縮至 NTA、Western blot、TEM、蛋白定量前可用體積
- 載藥 Exosome 的製備與濃縮
- 下游凍乾前的 Sample Prep
CentriVap Pro 能在這些流程中提供穩定、高效且低損傷的處理。
＃為什麼 EV 研究人員需要 CentriVap Pro？
- 溫和濃縮/精準控溫，不破壞外泌體結構
- 流程獲得完整的監控及高再現性
- 多樣式轉子搭配
- .可銜接上下游設備 (如 Labconco FreeZone 凍乾機）
Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems
The supernatant was dried using a CentriVap concentra-tor (VWR Labconco) 濃縮乾燥 and reconstituted in 50 μL of 75%methanol. Rb1 quantification was performed using LC–MS/MS
Source:
Jin, X., Wuyun, T., Zhang, Y., Wang, X. & Zhao, L.
Overcoming acquired immunotherapy resistance in non-small cell lung cancer using ginsenoside Rb1-loaded, peptide-enhanced exosome delivery systems.
J. Nanobiotechnol. 23, 443 (2025). https://doi.org/10.1186/s12951-025-03456-1
Composition of cardiac-derived extracellular vesicles changes with vesicle origin and determines uptake
Samples were stored at −80 0C until extraction. Lipids (脂質) were extracted from exosome (外泌體) samples with 400 µl of isopropanol (IPA). The samples were dried with a CentriVap Labconco (濃縮乾燥), reconstituted 50 µl IPA, vortexed mixed, sonicated for 5 min, and centrifuged at 21,100xg for 5 min.
Source:
Bheri, S. et al. Composition of cardiac-derived extracellular vesicles changes with vesicle origin and determines uptake. Front. Cardiovasc. Med.
12, 1565104 (2025).
https://doi.org/10.3389/fcvm.2025.1565104
Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages
Mass spectrometry analysis 質譜分析
To identify the proteins of exosome-like 類外泌體 vesicles from T. pisiformis cysticercus, three biological replicates were prepared as described above. Peptides were desalted with a Waters Oasis HLB column and eluted in 2% ACN and 0.1% FA before drying with a Benchtop Centrifugal Vacuum Concentrator 桌上型離心真空濃縮, (Labconco, Kansas City, MO, USA). Peptides were subjected to LC-20AD nano- HPLC spectrometry for peptide separation and data analysis.
Source:
Fu, Y. et al. Characterization of exosome-like vesicles derived from Taenia pisiformis cysticercus and their immunoregulatory role on macrophages.
Parasites Vectors 13, 318 (2020).
https://doi.org/10.1186/s13071-020-04177-3
