Ultra HPLC System Configuration
Ultra-HPLC uses the same separation methodology as conventional HPLC, but typically uses columns packed with particles smaller than 2μm. These smaller particles dramatically increase column efficiency, which in turn increases mass sensitivity, analytical resolution, and speed. These attributes are important for such applications as proteomics and pharmaceutical samples.
While instrumentation for Ultra-HPLC requires com- ponents similar to conventional HPLC, smaller column particle size results in higher system pressures and requires components, such as pumps, with high pres- sure ratings. In addition, the ideal pump should provide flow rates that are pulseless and stable. Teledyne Isco pumps are excellent Ultra-HPLC pumps.
Figure 1: System Configuration
